ELISA-Plate is a flat bottom polystyrene plate contains 96 or 384 wells holding 350 microliters each. Immunoassay is a laboratory technique use to bind between antigen and its homologous antibody to identify specific antigen or antibody in the sample. Antibodies are proteins produced in response to antigenic simulations. Antigen mean any molecule that elicit the production of antibodies when introduced into body. Adsorption is a process of adding antigen or/and antibody and samples with dilution buffer to soldi phase through incubation. Enzyme Conjugate mean enzyme that attached irreversibly to antibody. Washing mean flooding ELISA wells with Phosphate Buffer Saline Tween (PBST) three times to separate bound from unbound reagents. ELISA Plate used for (Microbiology Test – Food Allergens Test – Diseases Test)
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