Moist Heat Sterilization: 1- Sterilization Below 100 degree Celsius such as Pasteurization that sterilize milk and juice to inactive harmful microorganisms , 2- Sterilization at 100 degree Celsius such as Water Bath sterilization that sterilize serum and vaccine at 60 degree Celsius for one hour several days and Boiling that sterilize physical objects in a boiling water at 100 degree Celsius for 10 to 30 minutes and Tyndalization or steaming by using boiling water vapor for two minutes three days sequences , 3- Sterilization above 100 degree Celsius such as Autoclave that sterilize physical items for 20 minutes to coagulate proteins and destroy functional integrity of cell membrane of microorganisms
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