Principle of CAPS and dCAPS Markers

To prepare 4% stacking gel in volume 4 millimeters, add 2.7 millimeters distilled water + 0.67 millimeters 30% acrylamide + 0.5 millimeters 1MTris with pH 6.8 + 0.04 millimeters 10% SDS + 0.04 millimeters 10% ammonium per sulfate + 0.004 millimeters TEMED, while, to prepare 10% running or separating gel in volume 10 ml, add 4 millimeters distilled water + 3.3 millimeters 30% acrylamide + 2.5 millimeters 1.5M Tris with pH 8.8 + 0.1 millimeters 10% SDS + 0.1 millimeters 10% ammonium per sulfate + 0.01 millimeters TEMED #geneticteacher