Functions of Taq DNA Polymerase in PCR Reaction

PCR Primers: 1- Serves as a start site for DNA polymerase which extend in 5 to 3 direction, 2-should be (18 – 25) nucleotides in length that bind to one strand of the DNA and giving specificity, 3- Two primers are needed (One for each strand of DNA), 4- Calculating melting temperature of PCR primer depending on nearly identical for both primers, 5- Good primer design is critical to the success of PCR amplification, 6- Taq DNA polymerase cant not bind in the absence of PCR primers. #geneticteacher