Eukaryotic gene structure consist of Upstream intergenic region and downstream intergenic region and transcribed region involved 5-UTR (5-untranslated region) and 3-UTR (3-Untranslated region) and exons and introns, therefore 3% of eukaryotic genome is coding, while, over 85% of prokaryotic genome coding, Splice sites have donor (almost always Guanine and Thymine) and acceptor (almost always Adenine and Guanine), Interphase between exons and introns called donor site, while interphase between introns and exons called acceptor site, Cap region nucleotides (represent first nucleotide of gene structure), TATA box nucleotides (located in approximate 25 base pair in upstream region), CCAAT box nucleotides (located in approximate 100 base pair., in upstream region), GC box nucleotides (located in approximate 200 base pair in upstream region)
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