Disrupt SDS-PAGE buffer (10% SDS + Glycerol + Betamercaptoethanol + 1M Tris-HCL with pH 6.8 + Bromophenol blue) heat at least 95 degree Celsius for 4 minutes, (Tris-HCL, provide appropriate pH), (SDS use as detergent to dissolve proteins and give them negative charge), (Glycerol, use to make samples sink into wells), (Bromophenol blue, consider a dye to visualize samples) #geneticteacher

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