Functions of Taq DNA Polymerase in PCR Reaction

PCR, is a technique sed to amplify DNA in minutes according to the following steps: 1- DNA is heated (denatured) to separated the strands of DNA at 95 degree Celsius, 2- Primers (short strands of DNA target specific regions of DNA for replication) by hybridize (Anneal) with the separated strands of DNA at 55 to 65 degree Celsius, 3- DNA polymerase directs the rebuilding of the double stranded DNA molecule using Taq DNA Polymerase and dNTPs at 72 degree Celsius (Repeated for 30 -40 times) to generate millions copies of double strand DNA, 4- Each strand of DNA used as a template to create replicate that permits doubling of the number of target molecules with each cycle of heating and cooling #geneticteacher