Polyacrylamide gel has a tight matrix, so it consider ideal for proteins separation, due to smaller pore size than agarose, because of proteins much smaller than DNA, so the average of amino acids= 110 Daltons and the average of nucleotide pair= 649 Daltons, hence 1 Kbp of DNA= 650 Daltons, while 1 Kbp of DNA encodes 333 amino acids= 36 kilo Daltons #geneticteacher

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