Typical Programs of PCR Procedures: 1- Denaturation Phase (Denature template strands at 94 degree Celsius for (2 – 5) minutes, 2- Annealing Phase (Temperature variables for specific primers), so this phase is the most critical one for getting successful PCR reaction, 3- Extension Phase (generally at 72 degree Celsius) this is operating temperature for many thermos stable DNA polymerases
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