Functions of Taq DNA Polymerase in PCR Reaction

Stocks of gel solutions of SDS-PAGE: Stock 1 (30 gm acrylamide + 0.8 gm Bis-acrylamide) complete total volume to 100 ml distilled water, Stock2 (18.2 gm Tris-base or Tris-HCL dissolved in 50 ml distilled water) complete total volume to 100 ml distilled water with pH= 8.8, Stock 3 (6 gm Tris-HCL dissolve in 50 ml distilled water) complete total volume to 100 ml distilled water with Ph= 6.8 #geneticteacher