Samples buffers of SDS-PAGE: 7.88 gm Tris-HCL dissolved in 50 ml distilled water to make solution 1 molar Tris-HCL with pH= 6.8, then, mix them with (1 gm SDS + 2.5 ml Tris-HCL 1 molar) dissolved in 40 ml distilled water and complete total volume to 50 ml
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