Contents of samples buffer of SDS-PAGE: 1- SDS, denature proteins and makes them negatively charged, 2- B-mercaptoethanol, use to break disulphide bonds, 3- Glycerol, increase density of sample relative to surrounding running buffer making it easier to load in well, 4- Bromophenol blue, use to follow the run proteins samples on gel as a tracking dye #geneticteacher

Comments