Functions of Taq DNA Polymerase in PCR Reaction

Protein extracted by grinding 1 gm freeze dried sample in pestle and mortar with liquid nitrogen to crush samples, the electrode buffer is placed on them and transferred to Eppendorf tube, boiled for 10 minutes and centrifuge at 10000 rpm to take the supernatant as isolated protein,, then estimate the protein content in supernatant with bovine serum albumin as standard protein, then adjust protein content to 2 mg /ml #geneticteacher