GENETIC TEACHER

Chemical Fume Hood Cabinet Precautions: 1- Raise sash in lowest work height for protection, 2- Place equipment's in at least 12 inches from hood sash, 3- Clean 50% inside hood for work, 4- Work in at least 6 inches inside hood to ensure all vapors and gases trapped in hood, 5- Don’t store chemicals inside hood long period, 6- Store chemicals in a appropriate cabinet and bring them into fume hood as needed, 7- Don’t place head inside hood, 8- Stop work once alarm run, 9- Clean hood before and after work, 10- Close sash after finished #geneticteacher

Parts of Chemical Fume Hood Cabinet: 1- Light switch (Switch ON/OFF), 2- Sash (Glass window protect user during work), 3- Baffles (Located in hood back to uniform airflow), 4- Airfoil (Less airflow), 5- Work Surface (Lab bench inside hood), 5- Exhaust (Outside ventilate system) #geneticteacher

Chemical Fume Hood Cabinet operate by pulling air from lab space room into fume hood and out of building using sash in lowest to reduce chemical vapors to protect lab workers from inhaling toxic gasses and flammable chemicals #geneticteacher

Applications of Laminar Airflow Cabinets: 1- Life Sciences Researches, 2- Microbiology Researches, 3- Histopathology Researches, 4- Pathology Researches, 5- Plant Tissue Culture Researches, 6- Cell Culture Researches, 7- Electrons sectors, 8- Industrial sectors #geneticteacher

Laminar airflow Cabinet Precautions: 1- Only arms and Hands allowed inside work zone, 2- Don’t overload cabinet with unnecessary items, 3- Make sure all items are disinfected, 4- Laminar Airflow Cabinet doesn't use for storage infectious materials, 5- Start work from left to right direction inside Laminar Airflow Cabinet #geneticteacher

To avoid contamination: 1- Don’t touch feet with floor, 2- Place cabinets in at least (1-3) meters distance from door or other sides, 3- Avoid high traffic area (people walking) in front of cabinet #geneticteacher

Before cleaning Laminar Airflow Cabinet: 1- Wear gloves and lab coat for protection from hazardous substances, 2- Remove any equipment's and supplies and debris from workstation #geneticteacher

During work with Laminar Airflow Cabinet: 1- Clean area around cabinet to avoid dust inside filters, 2- Open sash and clean all inner surfaces with 70% ethanol alcohol, 3- Turn On fan, 4- Run Airflow for 15 minutes, 5- Close sash tightly and Turn ON UV lamp for 15 minutes, 6- Turn OFF UV lamp, 7- Turn ON Fluorescent lamp, 8- Start work now, 9- After completing (Turn OFF cabinet), 10- Use 70% ethanol alcohol or general disinfection #geneticteacher

In Vertical Laminar Airflow Cabinet Air directed through HEPA filter from top to bottom to doesn't blow air directly toward user to provide best work place by decreasing movement of contaminated air. In Horizontal Laminar Airflow Cabinet Air directed through HEPA filter from back workstation toward user to create ultra sterilization inside cabinet with constant velocity #geneticteacher

Parts of Laminar Airflow Cabinet: 1- Body (Provide isolation inside cabinet from external environment), 2- Sash (Front glass door), 3- Tabletop (Flat workstation made of stainless steel), 4- HEPA Filter (offer 99.99% sterilization efficiency through 0.3 micron), 5- Blower (Assembly room air toward HEPA filter), 6- Fan (Moves filtered air inside cabinet), 7- Ultraviolet Lamp (Sterilize elements inside cabinet before work), 8- Fluorescent Lamp (Provide proper light during work) #geneticteacher

Laminar Airflow Cabinet is enclosed bench work in combination with HEPA filter (Air passed through HEPA filter to remove all airborne contamination) to create aseptic conditions without risk contamination for biological samples and any particles sensitive materials. #geneticteacher

There are 4 classes of fire: Class A Like Ash (paper and wood and clothes and rubbers and plastics) (Overcome by Water and Dry Chemical), Class B Like Flammable and Gasses (Gasoline and Kitchen) (Overcome by Carbon Dioxide and Dry Chemical and Halon), Class C like Electrical and wiring equipment’s (Motors and Computers) (Overcome by Carbon Dioxide and Dry Chemical and Halon), Class D like Exotic Metals (Magnesium and Sodium and Titanium) (Overcome by Metal L-X (Sand)) #geneticteacher

Common Disinfected Chemical: 1- Bleach (1:10) (Stability to 7 days) (Apply for liquid waste and spills), 2- Phenolic (1:128) (Stability to 14 days) (Apply for surfaces), 3- Ethanol (70%) (Stable once time) (Apply for biosafety cabinet surfaces). But don’t autoclave any chemicals with bleach and don’t mix bleach with other disinfectants chemicals #geneticteacher

To reduce airflow disruption during work with biosafety cabinet: 1- Minimize hand and arm motions inside and outside cabinet, 2- Restrict foot traffic around cabinet, 3- Restrict open flames in cabinet #geneticteacher

Biosafety Cabinet Workflow: 1- Turn ON cabinet before work to let fan run for minutes, 2- Wipe down cabinet with 70% ethanol before and after use, 3- Wipe materials with 70% ethanol before bringing into cabinet, 4- Work from clean to dirty side #geneticteacher

Biological Safety Cabinet (BSC) use to minimize personnel exposure when working with biological materials, and dividing to three classes (Class I + Class II + Class III) based on biological agents and risk degree. Class I cabinet exhaust 70% and recirculate 30% of air to provide only personal and environmental protection (Suitable for microbiology researches). Class II cabinet provide personal environment and product protection (Divided in types (A1, A2, B1 and B2) so it exhaust 70% and recirculate 30% of air except type B2 have 100% exhaust without air recirculate (Suitable for microbiological and biomedical researchers). Class II cabinet provide highest degree of protection for personal, environment and products because of it enclosed totally with arm length with gloves to attach manipulated agents inside cabinet (Suitable for highest sensitive researchers) #geneticteacher

Chemical Hazards such as (Reactives + Toxins + Flammables + Corrosives chemicals). Physical Hazards such as (Noise + Projectiles + Heating Devices + Slipping + Moving Machinery Accidents). Electrical Hazards such as (Fire + Shock + Unsafe use of extension cords + Malfunctioning Electric Equipment's) #geneticteacher

Biosafety Rules are techniques for personal prevention from lab agents and environmental exposure by wearing all times goggles and lab coats and gloves and lab shoes, and disinfect workspace with 70% ethanol without food and drink and smoke and mobile and sandal #geneticteacher

Biosafety (Level-1) agents don’t cause disease. Biosafety (Level-2) agents associated with human disease. Biosafety (Level-3) indigenous exotic agents associated also with human disease. Biosafety (Level-4) dangerous exotic agents of life threatening nature #geneticteacher

Biosafety Levels: 1- Biosafety (Level-1) (Doesn’t cause disease for lab workers + Require minimum safety such as gloves and masks + No special practices or jut use standard procedures), 2- Biosafety (Level-2) (Moderate individual risk with low community risk) (Cause human disease for workers + Need gloves and facial protection and apron + Need Biosafety Cabinet + Need Autoclave Glassware), 3- Biosafety (Level-3) (High individual risk with low community risk) (Cause serious human disease for workers + Need gloves and facial protection and apron + Need Biosafety Cabinet + Need Autoclave Glassware), 4- Biosafety (Level-4) (High individual risk with high community risk) (Cause serious and lethal human disease for workers + Require positive pressure suit with segregated air supply + Need specific workers) #geneticteacher

There are two steps of risks: 1- Risk Assessment such as Assess Hazards and Identify Hazards, 2- Risk Managements such as Make Risk Decision and Develop and Implement controls #geneticteacher

Biohazard or Biological hazards refer to organism or organic matters produced by organisms that are harmful to human health in form of infections such as Bacteria or Virus or Parasites or Fungi #geneticteacher

Application of Atomic Absorption Spectroscopy: 1- Biological Samples such as Blood and Tissue, 2- Environmental Samples such as Water and Soil and Plants, 3- Industrial Samples such as Foods and Pharmaceuticals #geneticteacher

The Mechanisms of Atomic Absorption Spectroscopy: 1- Lamp (emits light toward element of interest from Hollow Cathode Lamp or Electrodeless Discharge Lamp, 2- Atomizer (Converts liquid sample into free atoms from energy absorbed from lamp, 3- Monochromator (send UV light wavelength for measurement, 4- Detector (Measure absorbed UV light wavelength that is emission from Monochromator), 5- Readout System (Computer and Printer) #geneticteacher

Atomic Absorption Spectroscopy measures concentrations of elements in parts per million of grams by expose samples to flams or high temperature #geneticteacher